EC Number |
Protein Variants |
Reference |
---|
1.13.11.B6 | A562G |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
724736 |
1.13.11.B6 | A562G |
the mutation does not affect the relative yield of 13-hydroperoxide, but increases the proportion of (13R)-enantiomer compared to the wild-type enzyme |
724736 |
1.13.11.B6 | F580A |
kcat/KM for linoleate hydroperoxidation is increased by 35% |
703535 |
1.13.11.B6 | F580A |
possesses activity profile that is similar to wild-type |
703535 |
1.13.11.B6 | F580V |
possesses activity profile that is similar to wild-type |
703535 |
1.13.11.B6 | G567A |
site-directed mutagenesis, the mutant converts the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation |
742627 |
1.13.11.B6 | G567A |
site-directed mutagenesis, the mutant converts the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation, mutant substrate specificity compared to the wild-type enzyme, chiral analysis |
742627 |
1.13.11.B6 | I437L |
KM-value for linoleate is 114% of wild-type value, kcat/Km for linoleate is 83% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.7 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
1.13.11.B6 | I566F |
site-directed mutagenesis, inactive mutant |
742627 |
1.13.11.B6 | I578L |
site-directed mutagenesis, mutant substrate specificity compared to the wild-type enzyme, chiral analysis |
742627 |