EC Number   |
Protein Variants |
Reference |
|---|
  1.13.11.72 | E176A |
site-directed mutagenesis, the mutant enzyme shows similar activity as the wild-type enzyme. Like the wild-type enzyme, the mutant HEPD-E176A produces hydroxymethylphosphonate and formate as its only detectable products upon incubation with Fe(II), hydroxyethylphosphonate, and O2 |
-, 745178 |
| 1.13.11.72 | E176H |
site-directed mutagenesis, the mutant is bifunctional exhibiting the activity of both 2-hydroxyethylphosphonate dioxygenase (HEPD) and methylphosphonate synthase (MPnS, EC 1.13.11.73). The product distribution of the mutant is sensitive to a substrate isotope effect, consistent with an isotope-sensitive branching mechanism involving a common intermediate. The introduced histidine does not coordinate the active site metal, unlike the iron-binding glutamate it replaces. More HEPD activity is observed when the reaction is carried out with (R)-2-[2-2H1]-hydroxyethylphosphonate |
-, 745162 |
| 1.13.11.72 | E176H |
the mutant catalyzes the transformation of 2-hydroxypropylphosphonate to methylphosphonate |
-, 763810 |
| 1.13.11.72 | K16A |
loss of enzymic activity |
718907 |
| 1.13.11.72 | R90A |
large decrease in ratio kcat/Km, mutant cannot be saturated in O2 |
718907 |
| 1.13.11.72 | R90K |
slight decrease in ratio kcat/Km |
718907 |
| 1.13.11.72 | Y98F |
large decrease in ratio kcat/Km, mutant cannot be saturated in O2. Mutant produces methylphosphonate as a minor side product |
718907 |
