EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.60 | A891G |
site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme |
-, 741712 |
| 1.13.11.60 | C1005S |
the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation |
764229 |
| 1.13.11.60 | C1006S |
the hydroperoxide isomerase activity of 5,8-LDS mutant is abolished |
711024 |
| 1.13.11.60 | C1006S/V328F |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | C1006S/V328L |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | C1006S/V328S |
the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts |
711024 |
| 1.13.11.60 | C1038S |
site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE |
741712 |
| 1.13.11.60 | D749A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
| 1.13.11.60 | E384A |
mutant forms only traces of (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
704475 |
| 1.13.11.60 | E728A |
the mutant shows less than 10% activity of the wild type enzyme |
764229 |
