EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.18 | C161Y |
mutation reported in ethylmalonic encephalopathy patients, drastic reduction in iron content |
765046 |
| 1.13.11.18 | C201S |
site-directed mutagenesis, the mutant protein structure is similar to the wild-type, but it shows reduced activity |
741915 |
| 1.13.11.18 | C201S/C408S |
site-directed mutagenesis |
741915 |
| 1.13.11.18 | C2S |
site-directed mutagenesis, the mutant is larger than the wild-type and is a hexamer |
741915 |
| 1.13.11.18 | C314S |
site-directed mutagenesis, structure comparison with wild-type enzyme. C314S BpPRF exhibits a 29fold lower kcat and an 5fold higher Km for GSSH than the wild-type |
742906 |
| 1.13.11.18 | C408S |
site-directed mutagenesis, the mutant is larger than the wild-type and is a hexamer. Substitution of the presumed Rhod active-site C408 also appears to destabilize the native protein fold. Catalytically inactive mutant |
741915 |
| 1.13.11.18 | D130A |
mutation in putative iron ligand, 0.5-2% residual activity |
764755 |
| 1.13.11.18 | D130E |
mutation in putative iron ligand, 0.5-2% residual activity |
764755 |
| 1.13.11.18 | D130H |
mutation in putative iron ligand, 0.5-2% residual activity |
764755 |
| 1.13.11.18 | D141A |
inactive concomitant to a decrease of melting point by 3-8 K |
764755 |
