EC Number   |
Protein Variants |
Reference |
|---|
   1.11.1.18 | D229A |
inactive mutant protein |
672312 |
| 1.11.1.18 | H258Q |
inactive mutant protein |
672312 |
| 1.11.1.18 | H480A |
results in the loss of the ability to efficiently oxidize bromide, but retains the ability to oxidize iodide |
675246 |
| 1.11.1.18 | more |
chemical synthesis of mono- and dinuclear oxidovanadium(V) complexes of an amine-bis(phenolate) ligand with bromoperoxidase activities, synthetic routes and kinetics of the complexes, NMR studies, kinetics, and mechanism and catalytic cycle, overview. Complex [LV(O)(H2O2)] formed through the oxidation of Br- to Br+ by [LV(O)(O...Br) (H3O)]+ in the presence of H2O2/KBr, can satisfactorily catalyse the oxidative bromination of salicylaldehyde to give 5-bromosalicylaldehyde as a major product along with 3,5-dibromo-salicylaldehyde and 2,3,5-tribromosalicylaldehyde as minor products, in the presence of HClO4 in mixed solvents (H2O : MeOH : THF = 4 : 3 : 2) at 25°C. In the absence of the catalyst, the reaction mixture gives only a 4% conversion of salicylaldehyde to 5-bromosalicylaldehyde. Catalytic parameters for the bromination of salicylaldehyde by KBr/H2O2 (KBr = 40 mmol, H2O2 = 2 ml, i.e. 67 mmol) using two complexes as catalysts |
764543 |
| 1.11.1.18 | more |
synthesis, crystal structure, DFT calculations, protein interaction, anticancer potential and bromoperoxidase mimicking activity of oxidoalkoxidovanadium(V) complexes: DFT calculations, protein interaction analysis, circular dichroism study, anticancer activity determination with MCF-7 cells, and determination of mitochondrial membrane potential (MMP) as well as intracellular reactive oxygen species (ROS) production, detailed overview |
765476 |
| 1.11.1.18 | R379F |
increase in affinity for Br-, enables the mutant to oxidize Cl-, decrease in affinity for I- |
726122 |
| 1.11.1.18 | R379H |
decrease in affinity for Br- |
726122 |
| 1.11.1.18 | R397W |
the mutant shows a different behaviour on bromide binding compared to the wild type enzyme, (the bromide is missing the hydrogen-binding interaction that held it in the active site of the wild type enzyme) |
699389 |
| 1.11.1.18 | S97A |
inactive mutant protein |
672312 |
| 1.11.1.18 | S97C |
mutant with very low residual activity |
672312 |
