EC Number   |
Protein Variants |
Reference |
|---|
   1.10.3.11 | A216L |
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity |
726398 |
| 1.10.3.11 | A216N |
site-directed mutagenesis, the mutation results in almost complete loss of ubiquinol oxidizing activity |
726398 |
| 1.10.3.11 | C127S |
the substitution prevents oxidative inactivation of alternative oxidase and renders the protein insensitive to pyruvate activation, the mutated protein is instead activated specifically by succinate |
715002 |
| 1.10.3.11 | C172A |
site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme |
724413 |
| 1.10.3.11 | C78A |
the mutant shows significant (418%) stimulation by 5 mM succinate and little response to 5 mM pyruvate |
714299 |
| 1.10.3.11 | C78D |
the mutant is significantly stimulated (28%) by 5 mM succinate, pyruvate has no significant effect on the mutant enzyme activity |
714299 |
| 1.10.3.11 | C78E |
the mutant is significantly stimulated (37%) by 5 mM succinate |
714299 |
| 1.10.3.11 | C78K |
the mutant is insensitive to pyruvate or succinate but more active than the wild type without pyruvate |
714299 |
| 1.10.3.11 | C78L |
inactive |
714299 |
| 1.10.3.11 | C78Q |
inactive |
714299 |
