EC Number   |
Protein Variants |
Reference |
|---|
    1.1.3.15 | A95G |
enhanced activity with bulky substrates, increase in the reduction rate constants with substrates having a bulky alpha-substituent such as mandelate, when compared with wild-type enzyme |
440394 |
| 1.1.3.15 | A95G |
much enhanced oxidase activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid and L-mandelic acid |
440423 |
| 1.1.3.15 | E160G/V198I |
the half-life of the mutant lactate oxidase at 70°C is about 18times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 46fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 59fold higher than wild-type value |
673006 |
| 1.1.3.15 | E160G/V198I/G36S/T103S/A232S/F277Y |
the half-life of the mutant lactate oxidase at 70°C is about 2 times that of E160G/V198I mutant and about 36 times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 48fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 55fold higher than wild-type value. Mutant is obtained by direct evolution |
673006 |
| 1.1.3.15 | H260A |
site-directed mutagenesis |
698951 |
| 1.1.3.15 | H260Q |
site-directed mutagenesis |
698951 |
| 1.1.3.15 | more |
Arabidopsis thaliana plants overexpressing glycolate oxidase in chloroplasts accumulates both hydrogen peroxide and glyoxylate, enzyme-overexpressing plants show retarded development, yellowish rosettes, and impaired photosynthetic performance. The plants develop oxidative stress lesions under photorespiratory conditions but grow like wild-type plants under nonphotorespiratory conditions, phenotype, overview |
700815 |
| 1.1.3.15 | more |
co-expression of GOX with capsid protein P8 targets capsid protein P8 into peroxisomes: time course analysis demonstrates that the localization of capsid protein P8 in peroxisomes of Spodoptera frugiperda cells, co-expressing capsid protein P8 and GOX, changes from diffuse to discrete, punctuate inclusions during expression from 24 to 48 h post inoculation |
673694 |
| 1.1.3.15 | more |
construction and evaluation of a biosensor of chiral, biocompatible, conducting material comprising collagen I, silica gel and ferrocene involving glucose oxidase/lactate oxidase enzymes. Preparation of the ferrocene/collagen or ferrocene/collagen/silica modified electrodes. The material modifies and inverts the chiral selectivity of D-glucose oxidase and L-lactate oxidase in collagen incorporated in tetramethoxysilane, overview |
713216 |
| 1.1.3.15 | more |
enzyme knockout by Dicer-substrate small interfering RNAs (DsiRNAs) targeting hydroxyacid oxidase 1 (HAO1) mRNA, relationship of Hao1 mRNA and glycolate oxidase protein knockdown to oxalate reduction, overview |
-, 745815 |
