EC Number   |
Protein Variants |
Reference |
|---|
   1.1.2.7 | more |
a Ca2+-free enzyme mxaA mutant is inactive |
-, 684948 |
| 1.1.2.7 | more |
construction of Ca2+-lacking mutant enzymes MoxA-, MoxK-, and MoxL-, which possess a fully oxidized pyrroloquinoline quinone cofactor that is not in the semiquinone form, incubation in a calcium salt solution leads to full restoration of the mutant enzymes, the mutant enzymes show a cofactor binding defect and are insensitive to inhibitor cyclopropanol, comparison of wild-type and mutant enzyme structures, overview |
-, 684947 |
| 1.1.2.7 | more |
construction of Ca2+-lacking, catalytically inactive mutant apoenzymes, reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer |
684950 |
| 1.1.2.7 | more |
mutation of Cys103-Cys104, forming a disulfide brigde, leads to loss of catalytic activity |
685313 |
| 1.1.2.7 | more |
preparation of an inactive Ca2+-free MDH-containing Hyphomicrobium denitrificans strain, which contains a fully-oxidized pyrroloquinoline quinone cofactor, incubation of Ca2+-free MDH with Ca2+ ion leads to reconstituted, fully active enzyme containing fully-reduced, tightly bound PQQ, elucidation of the Ca2+ ion elimination mechanism, overview |
-, 673048 |
