EC Number |
Protein Variants |
Reference |
---|
1.1.1.378 | D200A |
site-directed mutagenesis, the mutant shows 16% activity with L-rhamnose compared to wild-type |
763033 |
1.1.1.378 | D200H |
site-directed mutagenesis, the mutant shows 22% activity with L-rhamnose compared to wild-type |
763033 |
1.1.1.378 | F99A |
site-directed mutagenesis, the mutant is inactive |
763033 |
1.1.1.378 | F99Y |
site-directed mutagenesis, the mutant is inactive |
763033 |
1.1.1.378 | I196A |
site-directed mutagenesis, the mutant is inactive |
763033 |
1.1.1.378 | Q156A |
site-directed mutagenesis, the mutant is almost inactive |
763033 |
1.1.1.378 | R15T |
site-directed mutagenesis, the mutant of AvRhaDH shows a Km value for NADP+ decreased by 116fold, which increases the ratio of NADP+ to NAD+ (0.188) 157fold, from that of the wild-type enzyme (18.5) |
763033 |
1.1.1.378 | S37H |
site-directed mutagenesis, the mutant shows an altered ratio of NADP+ to NAD+ (0.101) compared to that of the wild-type enzyme (18.5) |
763033 |
1.1.1.378 | T191F |
site-directed mutagenesis, the mutant shows 3.9% activity with L-rhamnose compared to wild-type |
763033 |