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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.236more enzyme suppression in potato plants by RNA interference (RNAi) and enzyme overexpression. The overexpression and gene knockdown of StTRII alters tropinone reduction in vivo, and has great influence on calystegine accumulation, in contrast to genetic manipulation of isozyme St-TRI, EC 1.1.1.206, phenotypes, calystegine analysis, overview. A strong reduction of calystegines appears in the StTRII RNAi lines 741221
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.236Y201V site-directed mutagenesis, the mutant enzyme shows restored activity with tropinone, while the wild-type is inactive with tropinone. The mutant enzymes also remain to have the ability to reduce the 3-quinuclidinone and 4-methylcyclohexanone, although the Vmax of DnTR2-Y201V toward 4-methylcyclohexanone and 3-quinuclidinone are significantly lower (23 and 3fold decrease, respectively) than that of wild-type DnTR2 protein 740901
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