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EC Number
Protein Variants
Commentary
Reference
6.5.1.3
D120A
alanine-scanning mutagenesis
652387
6.5.1.3
D135A
about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme
662241
6.5.1.3
D244A
site-directed mutagenesis
652429
6.5.1.3
D292A
no ligation activity
662241
6.5.1.3
D292E
about 10% of the ligation activity of wild-type enzyme
662241
6.5.1.3
D308A
about 70% of the ligation activity of wild-type enzyme
662241
6.5.1.3
D425N
lethal mutation
676215
6.5.1.3
D65A
inactive
748777
6.5.1.3
D726A
lethal mutation
676215
6.5.1.3
D95A
the mutant shows RNA 3'-P guanylylation activity
748777
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