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Results 1 - 10 of 17 > >>
EC Number
Amino acid exchange
Commentary
Reference
D178E
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable
D178N
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable
E125D
mutation in subunit B, molecular dynamics simulations
E125Q
mutation in subunit B, molecular dynamics simulations
K236E/E328A
mutant used for crystallization, secondary structure contents and enzymatic activities similar to wild-type
K254E
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable
K88R
mutation in subunit B, molecular dynamics simulations
more
activity of the engineered enzyme variants indicates that the acceptor stem loop is the principle discrimination element because insertion of this loop alone enhances the specificity of the archaeal enzyme toward tRNAGln2
more
construction of an an N-terminal deletion mutant lacking amino acids 1-186 corresponding to the eukaryote-specific protein domains. The domains substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Results 1 - 10 of 17 > >>