EC Number   |
Protein Variants |
Reference |
|---|
  5.5.1.B7 | F251A |
the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with a 3fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate |
759060 |
| 5.5.1.B7 | F251G |
the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme |
759060 |
| 5.5.1.B7 | F251S |
the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme |
759060 |
| 5.5.1.B7 | F251V |
the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with an 8fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate |
759060 |
| 5.5.1.B7 | F255A/N313A |
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol |
741179 |
| 5.5.1.B7 | F255H |
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product |
741179 |
| 5.5.1.B7 | F357G |
the mutant produces syn-halima-(5,13E)-dienyl diphosphate from geranylgeranyl diphosphate |
775170 |
