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EC Number
Amino acid exchange
construction of an Escherichia coli strain that lacks phenylalanine hydroxylase, PhhA, and in which the expression of Pseudomonas aeruginosa PhhA plus the recycling enzyme pterin 4a-carbinolamine dehydratase PhhB, rescues tyrosine auxotrophy. This rescue is abrogated by deleting folX or folM and restored by expressing the deleted gene from a plasmid. The folX deletion selectively eliminates tetrahydromonapterin production, the mutant strain lacks tetrahydromonapterin
deletion of tyrA (making PhhA the sole source of tyrosine) and folX results in a strain prototrophic for tyrosine, whereas the DELTAtyrA DELTAfolX strain is auxotrophic
generation of a folX knockout mutant from strain SK6600
Results 1 - 3 of 3