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Results 1 - 5 of 5
EC Number Protein Variants Commentary Reference
Show all pathways known for 5.1.1.4Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.4C130S mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein 676832
Show all pathways known for 5.1.1.4Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.4C300S mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein 676832
Show all pathways known for 5.1.1.4Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.4R240W mutant enzyme shows similar kinetic constants for proline and hydroxyproline as compared to the wild-type enzyme 730678
Show all pathways known for 5.1.1.4Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.4W241F the mutant enzyme shows 5.3fold, 430fold, and 5.8fold lower kcat/Km values for trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, and cis-4-hydroxy-D-proline respectively, mainly due to a marked decrease in kcat values, whereas no significant effects are found in the kinetic constants of proline, suggesting that this (hydrophobic and bulky) tryptophan residue plays an importance role in the recognition of hydroxyproline (more hydrophilic and bulky than proline) -, 730678
Show all pathways known for 5.1.1.4Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.4more development of a Trypanosoma vivax specific PCR detection method based on the Trypanosoma vivax proline racemase (TvPRAC) gene. Forward and reverse primers are designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR is conducted on DNA extracted from different haemotropic pathogens.Analysis of the analytical sensitivity of the different PCR methods, method evaluation, overview. Usage of Trypanosoma vivax isolates from bovine specimens from Nigeria, Ethiopia and Venezuela -, 749144
Results 1 - 5 of 5