EC Number   |
Protein Variants |
Reference  |
|---|
   4.2.3.9 | Y92V |
the mutant produces the alicyclic beta-(E)-farnesene as the major product |
644241 |
| 4.2.3.9 | Y92F |
the mutant enzyme is approximately 0.1% as active as the nonmutated recombinant enzyme, the mutant releases significant amounts of germacrene A and also produces various amounts of a further five hydrocarbons of molecular weight 204, valencene, beta-(E)-farnesene, alpha-selinene, beta-selinene and selina-4,11-diene. The CD spectrum of the mutant enzyme is very similar to that of the wild-type enzyme |
644242 |
| 4.2.3.9 | Y92A |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene. The relative amounts of linear products formed depend linearly on the size of the residues at position 92. ASY92A produces almost 80% of alicyclic sesquiterpenes and no aristolochene |
644243 |
| 4.2.3.9 | Y92A |
turnover number is approximately 2 orders of magnitude lower than the value observed for the wild-type enzyme,the mutant enzyme produces almost 80% of the alicyclic sesquiterpenes (E)-beta-farnesene and (E,E)-alpha-farnesene. The mutant also produces small amounts of additional hydrocarbons with a molecular weight of 204: alpha-selinene, beta-selinene, selina-4,11-diene, (E,Z)-alpha-farnesene, and beta-bisabolene. Km-value for trans, trans-farnesyl diphosphate is 0.0834 mM compared to 0.0023 mM for the wild-type enzyme |
644243 |
| 4.2.3.9 | Y92C |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene. Mutant ASY92C still produces about 6.8% of aristolochene |
644243 |
| 4.2.3.9 | Y92C |
turnover number is approximately 2 orders of magnitude lower than the value observed for the wild-type enzyme. Km-value for trans, trans-farnesyl diphosphate is 0.05027 mM compared to 0.0023 mM for the wild-type enzyme |
644243 |
| 4.2.3.9 | Y92F |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene |
644243 |
| 4.2.3.9 | Y92V |
reduction of the size of the side chain of residue 92 leads to the production of the alicyclic sesquiterpenes (E)-beta- and (E,E)-alpha-farnesene |
644243 |
| 4.2.3.9 | F178V |
wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene. Mutant enzyme produces 10.8% aristolochene, 54.1% germacrene, 5.2% valencene, 5.7% alpha-selinene, 9.1% beta-selinine, 9.2% (E)-beta-farnesene and 2.7% (E,E)-alpha-farnesene. kcat is 1429fold lower than wild-type value, Km-value is 4.9fold higher than wild-type value |
664584 |
| 4.2.3.9 | F178Y |
wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene. Mutant enzyme produces 86.4% aristolochene, 10.7% germacrene, and 2.7% valencene. kcat is 30fold lower than wild-type value, Km-value is 2.2fold higher than wild-type value |
664584 |
