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Results 1 - 10 of 12 > >>
EC Number
Amino acid exchange
Commentary
Reference
E243A
site-directed mutagenesis, Ca2+-binding residue, about 3.6fold reduced activity compared to the wild-type enzyme
E243A/E245A
site-directed mutagenesis, Ca2+-binding residue, about 4fold reduced activity compared to the wild-type enzyme
E245A
site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme
E333A
PCR site-directed mutagenesis, E333 is a key residue in catalysis, reduced activity
H272A
PCR site-directed mutagenesis, H272 is a key residue in catalysis, reduced activity
K250A
PCR site-directed mutagenesis, inactive mutant
N213Q
site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme
R271A
PCR site-directed mutagenesis, R271 is a key residue in catalysis
R271E
site-directed mutagenesis, active site mutant, catalytically inactive
R271K
site-directed mutagenesis, active site mutant, about 10fold reduced activity compared to the wild-type enzyme
Results 1 - 10 of 12 > >>