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construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique
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expression in transgenic Nicotiana tabacum BY2 cells increases the osmotolerance and growth rate of the cells under hyperosmotic conditions
C105A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
C105S
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
D91A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E115A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E115D
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E57A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E57D
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
F107A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme