EC Number   |
Protein Variants |
Reference  |
|---|
   4.1.99.5 | A134F |
site-directed mutagenesis, the mutant has the same global architecture as wild-type enzyme, the mutant shows highly reduced activity with the majority of long-chain aldehyde substrates tested. the A134F variant displays an approximate fourfold increase in the rate of butanal consumption and approximately sixfold increase in pentanal consumption compared to wild-type enzyme, the mutant generates enhanced levels of propane production in whole-cell biotransformations compared to wild-type cADO |
-, 727312 |
| 4.1.99.5 | more |
alteration of the enzyme's substrate specificity by engineering of active site residues involved in substrate binding, residues V41 and A134, adjacent to the C9 position of the ligand, might influence fatty acid binding, overview |
-, 727312 |
| 4.1.99.5 | V41Y |
site-directed mutagenesis, the mutant has the same global architecture as wild-type enzyme, the mutant shows highly reduced activity with the majority of long-chain aldehyde substrates tested |
-, 727312 |
| 4.1.99.5 | V41Y/A134F |
site-directed mutagenesis, the double mutant shows reduced activity with long-chain aldehyde substrates and increased activity with short-chain aldehyde substrates like the single mutants |
-, 727312 |
| 4.1.99.5 | L194A |
site-directed mutagenesis, the mutant has kinetic properties very similar to the wild-type enzyme |
-, 746597 |
| 4.1.99.5 | A118F |
site-directed mutagenesis, the mutant shows increased activity with n-butanal compared to the wild-type enzyme. A118F does not show any obvious activity against C14,16,18 aldehydes, and only exhibits slight activity towards n-dodecanal and n-decanal for long-chain substrates |
747351 |
| 4.1.99.5 | A121F |
site-directed mutagenesis, the mutant shows increased activity with C4,6,7 aldehydes compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | C70F |
site-directed mutagenesis, the mutant shows increased activity with n-hexanal compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | F87Y |
site-directed mutagenesis, the mutant shows increased activity with n-decanal compared to the wild-type enzyme |
-, 747351 |
| 4.1.99.5 | G31F |
site-directed mutagenesis |
747351 |
