EC Number   |
Protein Variants  |
Reference |
|---|
   4.1.99.5 | A118F |
site-directed mutagenesis, the mutant shows increased activity with n-butanal compared to the wild-type enzyme. A118F does not show any obvious activity against C14,16,18 aldehydes, and only exhibits slight activity towards n-dodecanal and n-decanal for long-chain substrates |
747351 |
| 4.1.99.5 | A121F |
site-directed mutagenesis, the mutant shows increased activity with C4,6,7 aldehydes compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | A134F |
site-directed mutagenesis, the mutant has the same global architecture as wild-type enzyme, the mutant shows highly reduced activity with the majority of long-chain aldehyde substrates tested. the A134F variant displays an approximate fourfold increase in the rate of butanal consumption and approximately sixfold increase in pentanal consumption compared to wild-type enzyme, the mutant generates enhanced levels of propane production in whole-cell biotransformations compared to wild-type cADO |
-, 727312 |
| 4.1.99.5 | A134F |
site-directed mutagenesis, the mutant shows slightly increased activity compared to wild-type |
-, 748564 |
| 4.1.99.5 | C107A |
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme |
-, 749051 |
| 4.1.99.5 | C107A/C117A |
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme |
-, 749051 |
| 4.1.99.5 | C117A |
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme |
-, 749051 |
| 4.1.99.5 | C70F |
site-directed mutagenesis, the mutant shows increased activity with n-hexanal compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | C71A |
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme |
-, 749051 |
| 4.1.99.5 | C71A/C107A |
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant |
749051 |
