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Amino acid exchange
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genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine
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