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Results 1 - 8 of 8
EC Number
Amino acid exchange
Commentary
Reference
C14S
mutation of auxiliary cysteine residue present in the active site, significant resuction in kcat value
C7S
structure in complex with phosphate shows the formation of a covalent phospho-Ser7 adduct indicating that the crystallized mutant mimics the phospho-enzyme intermediate of the dephosphorylation reaction
C9A
substrate-trapping mutant, retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins
C9S
mutation of active site cysteine, complete loss of activity
D118A
complete loss of activity
F120A
60fold reduction of specific activity
T11I
18fold reduction of specific activity with phospho-arginine substrates, marked increasing in the activity toward phospho-tyrosine substrates
T11V
18fold reduction of specific activity with phospho-arginine substrates, marked increasing in the activity toward phospho-tyrosine substrates
Results 1 - 8 of 8