EC Number   |
Protein Variants |
Reference |
|---|
   3.7.1.4 | A126S |
site-directed mutagenesis |
735304 |
| 3.7.1.4 | A213S |
the mutant completely loses catalytic activity towards phloretin |
750528 |
| 3.7.1.4 | E164A |
the mutation completely abolishes the hydrolytic activity towards phloretin |
-, 750528 |
| 3.7.1.4 | E273A |
the mutation completely abolishes the hydrolytic activity towards phloretin |
-, 750528 |
| 3.7.1.4 | F218Y |
site-directed mutagenesis |
735304 |
| 3.7.1.4 | H118A |
the mutant completely loses catalytic activity towards phloretin |
-, 750528 |
| 3.7.1.4 | H133A |
the kcat value for phloretin is decreased at least by 1 order of magnitude, whereas the corresponding Km value is barely affected compared to the wild type enzyme |
750528 |
| 3.7.1.4 | H160A |
the mutant completely loses catalytic activity towards phloretin |
-, 750528 |
| 3.7.1.4 | H160F |
the mutant completely loses catalytic activity towards phloretin |
-, 750528 |
| 3.7.1.4 | H217A |
the mutant has a Km value increased by 3fold and a kcat value decreased by 6fold compared to the wild type enzyme |
750528 |
