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Results 1 - 10 of 15 > >>
EC Number
Amino acid exchange
Commentary
Reference
E295D
site-directed mutagesis, mutant kinetics compared to the wild-type, overview
E295S
by site-directed mutagenesis, the Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template
E295S/L322T
the double mutant is constructed using the E295S mutant as the template
E295S/L322T
the double mutant is constructed using the E295S mutant as the template; the mutations lead to a loss of enzyme activity against 1-aminocyclopropane-1-carboxylate
-
E295S/L322T
the mutations lead to a loss of enzyme activity against 1-aminocyclopropane-1-carboxylate
G44D
completely inactive, CD spectrum is identical to wild-type
K51T
crystal structure in complex with substrate
more
ACC deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells, e.g. of Cucumis melo var. cantaloupensis or Arabidopsis thaliana, by converting the direct precursor for ethylene, which inhibits gene transfer, into 2-oxobutanoate, overview
more
construction and complementation of acdS and lrpL mutant strains with wild-type strain D3 ACC deaminase, phenotypes, overview
more
construction of a disruption mutant deficient of enzyme activity by insertion of a tetracycline resistance gene into the the coding region of the chromosomal copy of the ACC deaminase gene
Results 1 - 10 of 15 > >>