EC Number   |
Protein Variants |
Reference |
|---|
   3.5.5.5 | A165F |
decreased degree of amide formation compared to the wild type enzyme and forms about 3% phenylglycine amide from (R,S)-phenylglycinonitrile. In contrast to the wild-type enzyme, the variant almost exclusively forms (R)-phenylglycine. This point mutation results in an almost complete stereoinversion of the reaction |
-, 755880 |
| 3.5.5.5 | A114F |
inactive |
710940 |
| 3.5.5.5 | G109F |
inactive |
710940 |
| 3.5.5.5 | Y54I |
inactive |
-, 718648 |
| 3.5.5.5 | Y54K |
inactive |
-, 718648 |
| 3.5.5.5 | Y54R |
inactive |
718648 |
| 3.5.5.5 | R128K |
mutant demonstrates low enantioselectivity (44.9% enantiomeric excess) as compared with the wild-type enzyme (52.7% enantiomeric excess) |
-, 755936 |
| 3.5.5.5 | R128E |
mutant displays no activity toward mandelonitrile |
755936 |
| 3.5.5.5 | A136Y/I168Y |
mutant enzyme produces (R)-mandelic acid with an enantiomeric excess value of 89.7%, an R-enantioselectivity higher than that obtained with the single mutations A136Y and I168Y |
755936 |
| 3.5.5.5 | A136Y |
mutant enzyme shows reversed selectivity and preferentially produces (R)-mandelic acid with an enantiomeric excess values of 66.7% |
-, 755936 |
