EC Number   |
Protein Variants |
Reference |
|---|
   3.5.3.18 | R40E/R98H |
about 95% of wild type activity, stable monomer |
652942 |
| 3.5.3.18 | C249S |
active-site Cys249 residue |
664074, 667726 |
| 3.5.3.18 | H162G |
active-site His162 residue |
664074 |
| 3.5.3.18 | C249S |
C249 is predominant target for S-nitrosylation |
653681 |
| 3.5.3.18 | C249S |
catalytically inactive |
667726 |
| 3.5.3.18 | H162G |
catalytically inactive |
667726 |
| 3.5.3.18 | C249S |
Cys249 as the catalytic nucleophile required for intermediate formation |
667675 |
| 3.5.3.18 | R40E-R98H |
exclusively monomeric, expressed in Escherichia coli at levels similar to the wild type |
652942 |
| 3.5.3.18 | N36D |
expressed in Escherichia coli at levels similar to the wild type |
652942 |
| 3.5.3.18 | C274A |
inactive and is not labeled by inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide |
712238 |
