EC Number   |
Protein Variants |
Reference |
|---|
   3.5.3.18 | C249S |
active-site Cys249 residue |
664074, 667726 |
| 3.5.3.18 | C249S |
C249 is predominant target for S-nitrosylation |
653681 |
| 3.5.3.18 | C249S |
catalytically inactive |
667726 |
| 3.5.3.18 | C249S |
Cys249 as the catalytic nucleophile required for intermediate formation |
667675 |
| 3.5.3.18 | C249S |
the C249S mutant and D66N mutant are both incapable of forming a covalent adduct when incubated with 2-hydroxymethyl-4-chloropyridine |
719651 |
| 3.5.3.18 | C274A |
inactive and is not labeled by inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide |
712238 |
| 3.5.3.18 | C274A |
no detectable activity |
687644 |
| 3.5.3.18 | C275A |
retains a kcat value about half of wild type protein |
687644 |
| 3.5.3.18 | D244N |
wild type and D244N DDAH variants both form covalent adducts upon incubation with 2-hydroxymethyl-4-chloropyridine, showing mass additions that are consistent with covalent attachment of one equivalent of hydroxymethylpyridine to each enzyme. These results also indicate that Asp244 is not essential for covalent modification to occur |
719651 |
| 3.5.3.18 | D66N |
the C249S mutant and D66N mutant are both incapable of forming a covalent adduct when incubated with 2-hydroxymethyl-4-chloropyridine |
719651 |
