Refine search

Search Engineering

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 27 > >>
EC Number
Amino acid exchange
Commentary
Reference
C249S
active-site Cys249 residue
C249S
active-site Cys249 residue; catalytically inactive
C249S
C249 is predominant target for S-nitrosylation
C249S
Cys249 as the catalytic nucleophile required for intermediate formation
C249S
the C249S mutant and D66N mutant are both incapable of forming a covalent adduct when incubated with 2-hydroxymethyl-4-chloropyridine
C274A
inactive and is not labeled by inhbitor N-(but-3-yn-1-yl)-2-chloroethanimidamide
C274A
no detectable activity
C275A
retains a kcat value about half of wild type protein
D244N
wild type and D244N DDAH variants both form covalent adducts upon incubation with 2-hydroxymethyl-4-chloropyridine, showing mass additions that are consistent with covalent attachment of one equivalent of hydroxymethylpyridine to each enzyme. These results also indicate that Asp244 is not essential for covalent modification to occur
D66N
the C249S mutant and D66N mutant are both incapable of forming a covalent adduct when incubated with 2-hydroxymethyl-4-chloropyridine
Results 1 - 10 of 27 > >>