EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.54 | G559E/G572E |
site-directed mutagenesis, crystal structure analysis, the mutant shows 14fold increaed Km for allophanate, and reduced substrate binding at the N-domain active site, but is catalytically active |
734220 |
| 3.5.1.54 | G559E/G572E |
site-directed mutagenesis, the mutant shows abolished activity |
-, 744532 |
| 3.5.1.54 | H488A |
site-directed mutagenesis |
752542 |
| 3.5.1.54 | K91A |
site-directed mutagenesis, inactive mutant |
669098 |
| 3.5.1.54 | more |
construction of the gene encoding C-terminally truncated AtzF mutant, AtzF467 |
733034 |
| 3.5.1.54 | more |
generation of isolated domains |
734220 |
| 3.5.1.54 | more |
generation of the KlUADELTABCCP construct via insertion a stop codon after base pair 5223 |
-, 744532 |
| 3.5.1.54 | more |
significantly higher concentrations of extracellular NH4-N are detected in the cultures along with overexpression of urea carboxylase (UC) and allophanate hydrolase (AH) genes. The bacterium forms biofilm, and displays swimming and swarming motilities in presence of urea. Additional glucose supply to urea boosts the colonization but ameliorates the media alkalization and ammonification through suppression of gene expressions encoding UC and AH |
-, 774125 |
| 3.5.1.54 | R307A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 733342 |
| 3.5.1.54 | R307M |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 733342 |
