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EC Number
Protein Variants
Commentary
Reference
3.5.1.119
D95A
inactive mutant enzyme
-
,
733084
3.5.1.119
E10A
mutation abolishes Dop activity both in vivo and in vitro
-
,
730405
3.5.1.119
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complementation of a Dop deletion strain with a variant deleted for the coding sequence for the 24 most conserved amino acids leads to a reduced pupylome. Smaller amino acid deletions in the Dop loop also results in decreased pupylome abundance. Deletions nearer to the amino terminus have greater decreases in pupylome levels. A strain producing Dop lacking the amino acids tryptophan, aspartate, tyrosine, glutamate, and valine has the most similar pupylome to the variant deleted for the coding sequence for the 24 most conserved amino acids
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,
772929
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