EC Number |
Protein Variants |
Reference |
---|
3.4.22.B75 | C745K |
mutation does not alter deconjugation rates in comparison with wild-type SENP7 |
715494 |
3.4.22.B75 | C979S |
site-directed mutagenesis |
732566 |
3.4.22.B75 | C992A |
site-directed mutagenesis, inactive catalytic mutant |
731715 |
3.4.22.B75 | F709W |
end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured |
715494 |
3.4.22.B75 | more |
Depletion of SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 (miSENP7) and a GFP mRNA to enable identification of the transfected cells |
732566 |
3.4.22.B75 | more |
enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7 |
731715 |
3.4.22.B75 | more |
knockdown strategy to deplete SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 and GFP mRNA to enable the identification of microRNA-expressing cells |
731614 |
3.4.22.B75 | more |
lentivirus mediated SENP7 enzyme knockdown |
731599 |
3.4.22.B75 | V713E |
mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity |
715494 |
3.4.22.B75 | V713E/C745K |
C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K) |
715494 |