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Results 1 - 10 of 10
EC Number
Amino acid exchange
Commentary
Reference
C745K
mutation does not alter deconjugation rates in comparison with wild-type SENP7
C979S
site-directed mutagenesis
C992A
site-directed mutagenesis, inactive catalytic mutant
F709W
end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured
more
Depletion of SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 (miSENP7) and a GFP mRNA to enable identification of the transfected cells
more
enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7
more
knockdown strategy to deplete SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 and GFP mRNA to enable the identification of microRNA-expressing cells
more
lentivirus mediated SENP7 enzyme knockdown
V713E
mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity
V713E/C745K
C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K)
Results 1 - 10 of 10