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Results 1 - 4 of 4
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.51C25A enzyme is inactive 638881
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.51E208A the mutant enzyme recognizes benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin less effectively than wild-type cruzain does, turnover of the substrate is comparable, suggesting that once bound, mutant cruzain processes benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarinat rates similar to that of the wild-type enzyme. Diminutions by 300fold and 200fold in the kcat/Km values of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and benzyloxycarbonyl-Arg-Ala-7-amido-4-methylcoumarin, respectively, are observed for the E208A mutant cruzain compared to that of the wild type, suggesting that ion pairing between Glu208 and P2 Arg is essential for recognition of these substrates. This is also reflected in values of Km, which are increased for benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin (10fold) and benzyloxycarbonyl-Arg-Ala-7-amido-4-methylcoumarin (4fold) 752781
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.51E219P variant with altered cleavage recognition site 638881
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.51more induction of RNAi against brucipain of Trypanosoma brucei does not cure mice from infection, however, 50% of these mice survive 60 days longer than uninduced controls. The ability of Trypanosoma brucei to cross an in vitro model of the human blood-brain barrier is also reduced by brucipain RNAi induction -, 700875
Results 1 - 4 of 4