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EC Number
Amino acid exchange
Commentary
Reference
C112S
site-directed mutagenesis, replacement of the free cysteine residue with serine improves the refolding yield of the recombinant enzyme by 50%. The heat stability of this C112S variant was also significantly improved by supercharging
E136Y
the mutant shows clearly reduced activity compared to the wild type enzyme
E136Y/R213L
the mutant shows about wild type activity
E173A
site directed mutagenesis
E173K
site directed mutagenesis
F144A
site directed mutagenesis
F144S
site directed mutagenesis
H24Q
the mutant shows slightly reduced activity compared to the wild type enzyme
K63A
site directed mutagenesis
K63E
site directed mutagenesis
Results 1 - 10 of 30 > >>