EC Number   |
Protein Variants |
Reference  |
|---|
   3.4.21.27 | more |
FXIa/PKA4 is made by replacing the FXI A4 domain with the A4 domain from prekallikrein. A dimeric version of FXI/PKA4 (FXI/PKA4-Gly326) is prepared as a control. Activated FXIa/PKA4 and FXIa/PKA4-Gly326 activate factor IX with kinetic parameters similar to that of FXIa. FXI/PKA4 and FXIa/PKA4-Gly326 have coagulant activity similar to FXI. FXIa/PKA4, FXIa/PKA4-Gly326 and FXIa have similar affinities for activated platelets. The dimeric proteins FXI and FXI/PKA4-Gly326 promote coagulation similarly, however, monomeric FXIa/PKA4 has greatly reduced activity. The activated monomeric FXIa/PKA4 activates factor IX poorly in the presence of activated platelets |
650724 |
| 3.4.21.27 | FXI/G326C |
mutant of FXI with Gly326 residue mutated to Cys326 |
667620 |
| 3.4.21.27 | K145A |
site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | K148A |
site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | K149A |
site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | R144A |
site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | R144A/K145A/R147A/K148A/K149A |
site-directed mutagenesis of an autolysis loop residues, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | R147A |
site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme |
683157 |
| 3.4.21.27 | C362S/C482S |
site-directed mutagenesis, the mutant shows increased platelet binding compared to the wild-type enzyme |
683160 |
| 3.4.21.27 | more |
chimeric recombinant enzyme mutants rFXI-PKA3 and rFXIa-PKA3, with fusion of FXI to the Apple 3 domain of prekallikrein, show unaffected platelet binding compared to the wild-type enzyme, overview |
683160 |
