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Protein Variants
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3.4.11.19
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enzyme consists of N-terminal domain DAP-A and C-terminal domains DAP-B and DAB-C. Coexpression of DAP-A plus DAP-BC results in a complex that copurifies on Ni-NTA-column and shows a 10fold increase in KM-value compared to wild-type. Mutant lacking gamma-loop residues 476-486, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity. Mutant lacking gamma-loop residues 476-486 plus mutation N275R, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity.
670823
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