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Results 1 - 10 of 16 > >>
EC Number
Amino acid exchange
Commentary
Reference
D1711N
introduction of a glycosylation site, reduced transport rate to the plasma membrane; introduction of potential N-glycosylation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
D311Y
the mutant enzyme is characterized by a lowered KM value for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside and increased transglycosylation when using aliphatic 1,3-diols or alcohols bearing a delta-hydroxy ketone function as acceptors. The transglycosylation/hydrolysis ratio in reactions catalyzed by the mutant is completely inversed and weak secondary hydrolysis is postponed, thus providing the basis for high transglycosylation yields (between 68 and 93%); the mutant shows increased catalytic efficiency for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside compared to the wild type enzyme. The transglycosylation ability of the mutant is improved in comparison to the wild type enzyme
E165D
reduced activity
E165Q
almost no activity
E233A
site directed mutagenesis
E254Q
inactive
E351S
site directed mutagenesis
E373D
reduced activity
E373Q
almost no activity
E483Q
inactive
Results 1 - 10 of 16 > >>