Search Engineering

3.2.1.145

E112A

site-directed mutagenesis, catalytically inactive mutant, the mutation eliminates the galactan hydrolysis activity of the enzyme while it does not disrupt its ligand binding ability

3.2.1.145

E112Q

site-directed mutagenesis, catalytically inactive mutant, the mutation eliminates the galactan hydrolysis activity of the enzyme while it does not disrupt its ligand binding ability

3.2.1.145

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construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity