EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.123 | D133A |
no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide |
751102 |
| 3.2.1.123 | D342A |
no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide |
-, 751102 |
| 3.2.1.123 | D62A |
hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide is 71.9% compared to wild-type enzyme. Hydrolysis of beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide is 83.9% compared to wild-type enzyme |
-, 751102 |
| 3.2.1.123 | delN148-G154 |
loop-deleted mutant Asn148-Gly154, the truncation of loop 4 from Asn148 to Gly154 may increase the space of the crowded sugar-binding cavity, resulting in enhanced activity toward beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide (163%) and decreased activity (46.8%) toward beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide |
751102 |
| 3.2.1.123 | E224D |
strain C9, severely reduced enzyme activity |
-, 646701 |
| 3.2.1.123 | E224Q |
strain C9, severely reduced enzyme activity |
-, 646701 |
| 3.2.1.123 | E233A |
glycosyl-enzyme intermediate trapped by soaking crystals of the general acid/base knock-out mutant E233A, with an activated sugar donor, 2,4-dinitrophenyl beta-D-lactoside |
680770 |
| 3.2.1.123 | E233D |
strain M-777, severely reduced enzyme activity |
-, 646701 |
| 3.2.1.123 | E233Q |
strain M-777, severely reduced enzyme activity |
-, 646701 |
| 3.2.1.123 | E234D |
strongly decreased enzyme activity compared with the wild-type enzyme |
680753 |
