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EC Number
Amino acid exchange
Commentary
Reference
D133A
no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide
D342A
no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide
D62A
hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide is 71.9% compared to wild-type enzyme. Hydrolysis of beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide is 83.9% compared to wild-type enzyme
delN148-G154
loop-deleted mutant Asn148-Gly154, the truncation of loop 4 from Asn148 to Gly154 may increase the space of the crowded sugar-binding cavity, resulting in enhanced activity toward beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide (163%) and decreased activity (46.8%) toward beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide
E224D
strain C9, severely reduced enzyme activity
E224Q
strain C9, severely reduced enzyme activity
E233A
glycosyl-enzyme intermediate trapped by soaking crystals of the general acid/base knock-out mutant E233A, with an activated sugar donor, 2,4-dinitrophenyl beta-D-lactoside
E233D
strain M-777, severely reduced enzyme activity
E233Q
strain M-777, severely reduced enzyme activity
E234D
strongly decreased enzyme activity compared with the wild-type enzyme
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