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Results 1 - 10 of 94 > >>
EC Number
Amino acid exchange
Commentary
Reference
C196T/C603T
double mutant to avoid restriction enzymatic scission
D19N/D22N/E25Q/D31N/D38N/E50Q/E57Q/E76Q/D77N/D79N/E92Q/D93N
site-directed mutagenesis using three different PCR primers
D1W
the mutant is studied in concentrated urea and GdnHCl solution with their disulfide bond broken, the results show that long-range effects in a denaturated protein can significantly effect the fluorescence properties
D303N
mutant with amino acid substitution in the binding pocket, inactive enzyme
D31N/D38N/E92Q/D93N
site-directed mutagenesis using two different PCR primers
D33A
Tm-value at pH 7.0 in Mops buffer is 16 °C lower than wild-type value. The stability of the mutant enzyme is 6 kcal/mol less than wild-type RNase Sa
D346N
mutant with amino acid substitution in the binding pocket
D54A
mutant is modeled in silico for gaining insight into modulations of diffusional association behavior, which is reflected in the association rate
D54A/E60A
mutant is modeled in silico for gaining insight into modulations of diffusional association behavior, which is reflected in the association rate
D79A
Tm-value at pH 7.0 in Mops buffer is 9.2 °C higher than wild-type value. The stability of the mutant enzyme is 3.3 kcal/mol less than wild-type RNase Sa
Results 1 - 10 of 94 > >>