EC Number   |
Protein Variants |
Reference |
|---|
   3.1.21.4 | D134N/K190N/E204V |
inactive |
730491 |
| 3.1.21.4 | D140A |
no detectable activity in dsDNA nicking, mutant R1 subunit/wild-type R2 subunit |
751757 |
| 3.1.21.4 | D140A/E167A/K169A |
no detectable activity in dsDNA nicking, mutant R1 subunit/wild-type R2 subunit |
751757 |
| 3.1.21.4 | D144A |
no detectable activity in ssDNA nicking, mutant R2 subunit/wild-type R1 subunit |
751757 |
| 3.1.21.4 | D146A |
no detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit |
751757 |
| 3.1.21.4 | D146A/E177A |
slight detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit |
751757 |
| 3.1.21.4 | D146A/E177A/K179A |
no detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit |
751757 |
| 3.1.21.4 | D148A |
only traces of DNA cleavage activity when used in large excess |
666483 |
| 3.1.21.4 | D148G |
binds DNA with 2times reduced affinity compared to wild-type protein |
666483 |
| 3.1.21.4 | D148G |
only traces of DNA cleavage activity when used in large excess |
666483 |
