EC Number |
Protein Variants |
Reference |
---|
2.8.3.18 | E294A |
complete loss of activity |
721675 |
2.8.3.18 | E294A |
site-directed mutagenesis, the mutant specific catalytic activity is 10000fold reduced compared to the wild-type enzyme, ligand bound crystal structure modeling |
721675 |
2.8.3.18 | E435A |
mutant protein is completely insoluble |
721675 |
2.8.3.18 | E435A |
site-directed mutagenesis, the mutant is completely insoluble, ligand bound crystal structure determination and analysis, the mutant crystallizes in a closed complex containing dethiaacetyl-CoA, which adopts an unusual curled conformation |
721675 |
2.8.3.18 | E435D |
activity similar to wild-type |
721675 |
2.8.3.18 | E435D |
site-directed mutagenesis, the mutant catalytic properties are nearly equivalent to those of the His6-tagged wild-type enzyme, ligand bound crystal structure modeling |
721675 |
2.8.3.18 | E435Q |
mutant protein is completely insoluble |
721675 |
2.8.3.18 | E435Q |
site-directed mutagenesis, the mutant is completely insoluble, ligand bound crystal structure modeling |
721675 |
2.8.3.18 | N347A |
large decrease in catalytic activity |
721675 |
2.8.3.18 | N347A |
site-directed mutagenesis, the mutant shows impaired catalytic activity, but the apparent affinities for all four substrates are largely unaffected, ligand bound crystal structure modeling |
721675 |