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Results 1 - 8 of 8
EC Number
Amino acid exchange
Commentary
Reference
E294A
complete loss of activity; site-directed mutagenesis, the mutant specific catalytic activity is 10000fold reduced compared to the wild-type enzyme, ligand bound crystal structure modeling
E435A
mutant protein is completely insoluble; site-directed mutagenesis, the mutant is completely insoluble, ligand bound crystal structure determination and analysis, the mutant crystallizes in a closed complex containing dethiaacetyl-CoA, which adopts an unusual curled conformation
E435D
activity similar to wild-type; site-directed mutagenesis, the mutant catalytic properties are nearly equivalent to those of the His6-tagged wild-type enzyme, ligand bound crystal structure modeling
E435Q
mutant protein is completely insoluble; site-directed mutagenesis, the mutant is completely insoluble, ligand bound crystal structure modeling
N347A
large decrease in catalytic activity; site-directed mutagenesis, the mutant shows impaired catalytic activity, but the apparent affinities for all four substrates are largely unaffected, ligand bound crystal structure modeling
N347A
the mutant retains about 15% of wild type specific activity
R228E
large decrease in catalytic activity; site-directed mutagenesis, the mutant has a specific defect in its ability to bind both carboxylate substrates, ligand bound crystal structure modeling
S71A
large decrease in catalytic activity; site-directed mutagenesis, the mutant shows impaired catalytic activity associated with lower kcat values, ligand bound crystal structure modeling
Results 1 - 8 of 8