EC Number   |
Protein Variants |
Reference |
|---|
    2.8.1.6 | H152A |
about 10% of turnover rate of wild-type. Ratio of 5-deoxyadenosine to biotin is about twice as high as in wild-type |
672076 |
| 2.8.1.6 | R260M |
contains 1.1 iron atoms per monomer, about 30% of wild-type activity |
672097 |
| 2.8.1.6 | R260H |
contains 1.3 iron atoms per monomer, slightly less active than wild-type |
672097 |
| 2.8.1.6 | R260A |
contains 1.8 iron atoms per monomer, slightly less active than wild-type |
672097 |
| 2.8.1.6 | R260C |
contains 2.0 iron atoms per monomer, slightly more active than wild-type |
672097 |
| 2.8.1.6 | C188A |
cysteine desulfurase activity is reduced to 70% of the wild-type activity |
645609 |
| 2.8.1.6 | C188S |
enzyme is insoluble |
645606 |
| 2.8.1.6 | more |
Fe/S cluster assembly of biotin synthase strongly depends on Isu1 and Isu2 proteins. Proteins Isa1 and Isa2 are crucial for in vivo function of biotin synthase but not for de novo synthesis of its Fe/S clusters |
-, 673421 |
| 2.8.1.6 | N153S |
inactive for biotin production, enzyme can bind AdoMet and DTB and can convert DTB to 9-mercaptodethiobiotin, the majority of 9- mercaptodethiobiotin is released into the buffer and not bound to the enzyme, suggesting that dissociation of 9- mercaptodethiobiotin is faster than the second half reaction for biotin formation |
691001 |
| 2.8.1.6 | C53A |
inactive, but still exhibits UV-visible spectrum of a [Fe2-S2] cluster similar to that of the wild-type enzyme |
645604 |
