EC Number |
Protein Variants |
Reference |
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2.7.8.43 | more |
construction of a membrane-deletion mutant of the enzyme |
-, 733017 |
2.7.8.43 | more |
construction of gene lptA mutant and complement strains from strain FA 1090 |
-, 733635 |
2.7.8.43 | more |
piliated colony variants of strain FA19 are transformed with plasmid or chromosomal DNA preparations bearing insertionally inactivated lpt3, lpt6, or lptA genes prepared previously from Neisseria meningitidis strain NMB |
-, 733963 |
2.7.8.43 | more |
construction of several enzyme mutant strains, overview |
-, 733966 |
2.7.8.43 | more |
construction of insertional lptA mutants from 35E (L2), H44/76 (L3), and 89I (L4) backgrounds |
734062 |
2.7.8.43 | more |
construction of a pmrA- null mutant strain JSG421 of covalently modified pmrAc strains JSG485 and JSG486, overview |
-, 734138 |
2.7.8.43 | more |
generation of polymyxin-sensitive mutants of an Escherichia coli pmrAC strain WD101 producing a lipid A that lacked both the 3'-acyloxyacyl-linked myristate (C14) and L-4-aminoarabinose, even though the necessary enzymatic machinery required to synthesize L-4-aminoarabinose-modified lipid A is present. Strain WD103 is generated by P1vir transduction of the polymyxin-resistant genotype (pmrAC) of strain WD101 into the Escherichia coli lpxM mutant MLK1067, phenotype, overview. The polymyxin-sensitive parent strains MLK1067 and W3110 produce unmodified penta- or hexa-acylated lipid A, respectively. Complementation of WD103 with the vector pWSLpxM resulted in restoration of polymyxin resistance to the levels displayed by WD101, effect of lipid myristoylation on the polymyxin resistance |
734151 |
2.7.8.43 | more |
generation of a soluble enzyme mutant by deletion of the membrane binding structure. The mutant does not confer resistance to polymyxin in transformed Escherichia coli cells, in contrast to the wild-type LptA |
-, 734476 |
2.7.8.43 | more |
JSG435 carries a mutant pmrA locus allele (pmrA505), JSG435 is mutagenized with the transposon Tn10d. Chromosomal mapping of the transposon insertions of the two polymyxin B sensitive strains shows them to be located at 50-52 centisomes (JSG485) and 43-45 centisomes (JSG486), thus eliminating the possibility that mutations in these regulatory loci are responsible for the observed phenotypic change |
-, 734706 |
2.7.8.43 | more |
generation of an lptA deletion mutant strain using plasmid pMEB256, with or without combined deletions of genes ptdA and ptdB |
735018 |