EC Number   |
Protein Variants |
Reference |
|---|
  2.7.7.B5 | more |
construction of two truncated forms of ThiI containing the N-terminal domain including NFLD and THUMP, and the C-terminal domain including the PP-loop and RLD domains, respectively. The N-domain can bind with both tRNAPhe and TPHE39A and recognizes the acceptor-stem region, whereas the C-domain cannot. The C-domain also affects RNA binding by its enthalpically favorable, but entropically unfavorable, contribution. The C-domain induces a conformation change in tRNAPhe |
710548 |
| 2.7.7.B5 | D189A |
mutation eliminates in vivo function of the enzyme |
691789 |
| 2.7.7.B5 | K321M |
mutation eliminates in vivo function of the enzyme |
691789 |
| 2.7.7.B5 | C456S |
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis |
725262 |
| 2.7.7.B5 | C207S |
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis |
725262 |
| 2.7.7.B5 | C344S |
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis |
725262 |
| 2.7.7.B5 | D189A |
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis |
725262 |
| 2.7.7.B5 | K321M |
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis |
725262 |
