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Results 1 - 10 of 15 > >>
EC Number
Amino acid exchange
Commentary
Reference
E448G
site-directed mutagenesis
G21A
mutant enzyme is degraded during the purification phase
G22A
mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 59% of that of the wild-type enzyme
G24A
mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 48% of that of the wild-type enzyme
G26A
mutant enzyme with decreased turnover-number/KmATP value
G27R
mutant enzyme is degraded during the purification phase. Turnover-number is 45% of that of the wild-type enzyme
K27E
mutant enzyme with 2600fold decreased turnover-number/KmATP value. Turnover-number is 21% of that of the wild-type enzyme
K27M
mutant enzyme with 1000fold decreased turnover-number/KmATP value. Turnover-number is 22% of that of the wild-type enzyme
more
construction of a UMP-CMPK2DELTA21 mutant lacking the mitochondrial targeting sequence
more
determination and analysis of 28 polymorphisms in DCK/CMPK enzymes, genotyping with humans of different ethnic origins, overview. Variant allozyme enzyme activities range from 32 to 105% of the wild-type enzyme for DCK and from 78 to 112% of wild-type enzyme for CMPK, with no significant differences in apparent Km values for either enzyme except for a DCK Val24/Ser122 double variant allozyme, overview
Results 1 - 10 of 15 > >>