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Results 1 - 10 of 21 > >>
EC Number
Amino acid exchange
Commentary
Reference
A38T
mutant shows increased resistance against glucosamine-6-phosphate
A602L
enhanced activity compared to the wild type enzyme, the behaviour of the mutant is similar to that of the wild type counterpart during purification demonstrating no significant modification in the overall protein structure
biotechnology
the recombinant enzyme expressed in Saccharomyces cereviaise reveals some differences from the wild type enzyme, such as improved stability and less sensitivity to UDP-GlcNAc
C1A
C1A-GlmS does not reveal glutaminase activity at 37°C when tested in the presence of Gln only
C1A
the structure of the inactive C1A mutant, crystallized in the presence of D-fructose 6-phosphate and Gln is deterimined. The C1A-GlmS structure is organized as a hexamer. The enzyme is regulated by a morpheein-type allosteric mechanism, in which functional dimeric GlmS is in equilibrium with the inactive hexamer
G40A
mutant with exchanged guanine is inactive. The 2.7 A resolution crystal structure of the mutant shows that the RNA is in a conformation nearly identical to that of the wild-type glmS ribozyme. The experimental electron density maps indicate that GlcN6P binds to the G40A mutant in the same location as in the wild-type ribozyme. Raman pH titrations of GlcN6P using crystals of the G40A mutant glmS ribozyme show that the pKa of the amine of the ribozyme-bound GlcN6P differs substantially for the wild-type and G40A mutant ribozymes
G471S
mutant shows increased resistance against glucosamine-6-phosphate
I271T
mutant shows increased resistance against glucosamine-6-phosphate
I3T
mutant shows increased resistance against glucosamine-6-phosphate
L468P
mutant shows increased resistance against glucosamine-6-phosphate
Results 1 - 10 of 21 > >>