EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.255 | V111G |
a naturally occuring 332 T > G missense mutant, the mutant Naa10 has a reduced stability and 85% reduced monomeric catalytic activity, while catalytic NatA function remains unaltered. NAA10-V111G has a reduced stability compared to wild-type NAA10, and in vitro acetylation assays reveal a reduced enzymatic activity of monomeric NAA10-V111G but not for NAA10-V111G in complex with NAA15 (NatA enzymatic activity). A glycine in position 111 instead of valine will not cause any steric clashes, but loss of the more bulky hydrophobic side chain of valine may possibly cause structural alterations affecting protein stability or acetyl-CoA binding |
756297 |
| 2.3.1.255 | I72T |
a naturally occuring mutation NAA10 c.215T>C, the mutant phenotype shows a milder phenotypic spectrum in comparison to most of the previously described patients with NAA10 variants. The three boys have development delay, intellectual disability, and cardiac abnormalities as overlapping phenotypes. NAA10 Ile72Thr protein is destabilized, while binding to NAA15 most likely is intact. The NatA activity of NAA10 Ile72Thr appears normal while its monomeric activity is decreased. Genotype-phenotype correlations for NAA10 variants, overview |
756642 |
| 2.3.1.255 | more |
construction of Naa10 stably knocked down H1299 cell line H1299-shNaa10, cDNA microarray analysis |
758388 |
| 2.3.1.255 | more |
enzyme knockdown by siRNA. Generation of mutant daf-31(m655) by removal of 151 bp of promoter upstream of the ATG start codon and 242 bp of daf-31 coding region dowstream of the ATG start codon. Generation of and overexpression mutant daf-31 OE for which the full-length dar-31 genomic DNA is cloned into pGEM-T vector. Generation of mutant vncBDk by with impaired N-terminal activity |
-, 756675 |
| 2.3.1.255 | more |
generation of an Ard1 null mutant by removal of the ARD1 coding region |
756675 |
| 2.3.1.255 | more |
generation of enzyme mutant by frame shift mutation causing a acetyltransferase-truncated enzymatic region, and of another mutant vnc by intron-insertion mutation |
756675 |
| 2.3.1.255 | more |
generation of mutant naa10-1 and of a naa15 mutant by T-DNA insertion-disrupting gene expression |
756675 |
| 2.3.1.255 | more |
generation of mutant naa10MO by morpholino-based knockdown |
756675 |
| 2.3.1.255 | more |
generation of mutants MtRimI4-158, MtRimI1-153, MtRimI4-153, MtRimIC21A, and of the final construct MtRimIC21A4-153, MtRimIC21A4-153 has almost identical enzymatic activity compared to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. The 2D 1H-15N heteronuclear single quantum coherence spectrum of tRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments show that MtRimIC21A4-153 possesses similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Structure comparison of wild-type MtRimI and mutant MtRimIC21A4-153 |
-, 755712 |
| 2.3.1.255 | more |
knockdown of Naa10p by shRNAs, knockdown efficiencies, overview. Generation of truncated Naa10p mutants |
756332 |
